Thermo Fisher Scientific, Biolyst and Heartland Biotech conducted a comprehensive, hands-on Volume EM workshop at the University of Louisville, designed to help researchers build practical skills across the complete workflow. Participants learned how to perform volume EM sample preparation in a wet-lab setting, explore unique and targeted stains as well as key immunolabeling considerations, and gain insight into how these choices support high-quality 3D imaging outcomes. Throughout the workshop, the PrepMaster® 5100 Automated Workflow System wsimultaneously processed equivalent specimens using the rOTO protocol, providing a side-by-side comparison of traditional manual methods and state-of-the-art laboratory automation.